ABSTRACT
OBJECTIVE:To determine the content of phenolic acids from Anemone altica,and optimize its extraction technolo-gy. METHODS:HPLC was used to determine the contents of mono-ferulyl tartaric acid and ferulic acid from A. altica;using the total contents of 2 index components as index,volume fraction of extraction solvent,extraction solvent volume,extraction times and extraction time as factors,orthogonal test was used to optimize extraction technology,and verification test was conducted. RE-SULTS:The contents of mono-ferulyl tartaric acid and ferulic acid were 0.059%,0.0025%,respectively;the optimal extraction technology was as follow as 30% ethanol 600 mL added to 20 g medicinal material,extracted twice,90 min every time. In verifi-cation test,the average contents of 2 components in extract were 0.2971%(RSD=3.64%,n=3),0.0041%(RSD=5.11%,n=3). CONCLUSIONS:A method for contents determination of mono-ferulyl tartaric acid and ferulic acid from A. altica is estab-lished;optimized extraction technology is stable and feasible.
ABSTRACT
Objective To optimize the conditions for the percolation extraction process of Panax notoginseng(Burk.) F.H.Chen.Methods Conditions for the percolation were studied by orthogonal experimental design as guided by the contents of total notoginseng saponin and total amino acids.Results The percolate rate,concentration and quantity of alcohol had significant effects on the process.Conclusion The optimum condition for the extraction of Panax notoginseng was adding 12 times amount of 50% alcohol and percolating at a rate of 1～3 mL/(min?kg).
ABSTRACT
Objective To establish a method for determination of oleanolic acid in Cortex Aralia Elatae by HPLC.Methods The determination was carried out with Kromasil C18 column(250 mm?4.6 mm,5 ?m),using acetonitrile-water-phosphoric acid(80∶20∶0.42) as the mobile phase and detected at the wavelength of 208 nm.Results Oleanolic acid showed a good linear relationship at the range of 1.164～5.820 ?g,r=0.999 9.The average recovery was 99.70% and RSD was 1.76%(n =5).Conclusion The method was simple and accurate with good reproducibility,and can be used to determine the content of oleanolic acid in Cortex Aralia Elatae.